Figure 5

Levels of NRP-1 and DBA expression are changed in the absence of adenylyl cyclase 3. (A-D) Coronal sections from AC3 heterozygous versus homozygous knock-out mice at E15. (A, B) NRP-1 (green)/NCAM (red)/DRAQ5 (blue); (C, D) DBA(green). (A, A') Coronal cross-section of the OB from a heterozygous AC3 mouse stained for NRP-1. (B-B') Coronal section from a homozygous AC3 knockout mouse stained for NRP-1. (A') High-magnification of boxed area in (A). (B') High-magnification of boxed area in (B). (C) Coronal section of the OB from a heterozygous AC3 knockout mouse stained for DBA. (D) Coronal section from a homozygous AC3 knockout mouse stained for DBA. An orientation compass is shown in (A'). Scale bars = 100 μm in (A-D) and 25 μm in (A'-D').